tetano
Editor, Senior Moderator
Viruses
. 2022 Feb 21;14(2):437.
doi: 10.3390/v14020437.
Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus
Daria Prokhorova[SUP] 1 2 [/SUP], Natalya Zhukova Eschenko[SUP] 1 [/SUP], Anna Lemza[SUP] 1 [/SUP], Mariia Sergeeva[SUP] 1 3 [/SUP], Rinat Amirkhanov[SUP] 1 [/SUP], Grigory Stepanov[SUP] 1 [/SUP]
Affiliations
Abstract
Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV replication are actively studied. One of the most convenient and accessible genome-editing tools to facilitate these studies is the CRISPR/Cas9 system. This tool allows for regulating the expression of both viral and host cell genes to enhance or impair viral entry and replication. This review considers the effect of the genome editing system on specific target genes in cells (human and chicken) in terms of subsequent changes in the influenza virus life cycle and the efficiency of virus particle production.
Keywords: CRISPR/Cas9; CRISPR/Cas9 genome screening; RNA processing factors; cell receptors; cellular immune response; influenza virus.
. 2022 Feb 21;14(2):437.
doi: 10.3390/v14020437.
Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus
Daria Prokhorova[SUP] 1 2 [/SUP], Natalya Zhukova Eschenko[SUP] 1 [/SUP], Anna Lemza[SUP] 1 [/SUP], Mariia Sergeeva[SUP] 1 3 [/SUP], Rinat Amirkhanov[SUP] 1 [/SUP], Grigory Stepanov[SUP] 1 [/SUP]
Affiliations
- PMID: 35216030
- PMCID: PMC8879999
- DOI: 10.3390/v14020437
Abstract
Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV replication are actively studied. One of the most convenient and accessible genome-editing tools to facilitate these studies is the CRISPR/Cas9 system. This tool allows for regulating the expression of both viral and host cell genes to enhance or impair viral entry and replication. This review considers the effect of the genome editing system on specific target genes in cells (human and chicken) in terms of subsequent changes in the influenza virus life cycle and the efficiency of virus particle production.
Keywords: CRISPR/Cas9; CRISPR/Cas9 genome screening; RNA processing factors; cell receptors; cellular immune response; influenza virus.