tetano
Editor, Senior Moderator
Viruses
. 2020 Aug 4;12(8):E849.
doi: 10.3390/v12080849.
Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by Mass Spectrometry
Petra Wandernoth[SUP] 1 [/SUP], Katharina Kriegsmann[SUP] 2 [/SUP], Cristina Groh-Mohanu[SUP] 1 [/SUP], Martin Daeumer[SUP] 3 [/SUP], Peter Gohl[SUP] 4 [/SUP], Oliver Harzer[SUP] 4 [/SUP], Mark Kriegsmann[SUP] 5 6 [/SUP], Joerg Kriegsmann[SUP] 1 7 [/SUP]
Affiliations
Abstract
Background: Amplification of viral ribonucleic acid (RNA) by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) is the gold standard to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the initial outbreak, strategies to detect and isolate patients have been important to avoid uncontrolled viral spread. Although testing capacities have been upscaled, there is still a need for reliable high throughput test systems, specifically those that require alternative consumables. Therefore, we tested and compared two different methods for the detection of viral PCR products: rRT-PCR and mass spectrometry (MS).
Methods: Viral RNA was isolated and amplified from oro- or nasopharyngeal swabs. A total of 22 samples that tested positive and 22 samples that tested negative for SARS-CoV-2 by rRT-PCR were analyzed by MS. Results of the rRT-PCR and the MS protocol were compared.
Results: Results of rRT-PCR and the MS test system were in concordance in all samples. Time-to-results was faster for rRT-PCR. Hands-on-time was comparable in both assays.
Conclusions: MS is a fast, reliable and cost-effective alternative for the detection of SARS-CoV-2 from oral and nasopharyngeal swabs.
Keywords: COVID19; SARS-CoV2; mass spectrometry; severe acute respiratory syndrome coronavirus 2.
. 2020 Aug 4;12(8):E849.
doi: 10.3390/v12080849.
Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by Mass Spectrometry
Petra Wandernoth[SUP] 1 [/SUP], Katharina Kriegsmann[SUP] 2 [/SUP], Cristina Groh-Mohanu[SUP] 1 [/SUP], Martin Daeumer[SUP] 3 [/SUP], Peter Gohl[SUP] 4 [/SUP], Oliver Harzer[SUP] 4 [/SUP], Mark Kriegsmann[SUP] 5 6 [/SUP], Joerg Kriegsmann[SUP] 1 7 [/SUP]
Affiliations
- PMID: 32759673
- DOI: 10.3390/v12080849
Abstract
Background: Amplification of viral ribonucleic acid (RNA) by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) is the gold standard to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the initial outbreak, strategies to detect and isolate patients have been important to avoid uncontrolled viral spread. Although testing capacities have been upscaled, there is still a need for reliable high throughput test systems, specifically those that require alternative consumables. Therefore, we tested and compared two different methods for the detection of viral PCR products: rRT-PCR and mass spectrometry (MS).
Methods: Viral RNA was isolated and amplified from oro- or nasopharyngeal swabs. A total of 22 samples that tested positive and 22 samples that tested negative for SARS-CoV-2 by rRT-PCR were analyzed by MS. Results of the rRT-PCR and the MS protocol were compared.
Results: Results of rRT-PCR and the MS test system were in concordance in all samples. Time-to-results was faster for rRT-PCR. Hands-on-time was comparable in both assays.
Conclusions: MS is a fast, reliable and cost-effective alternative for the detection of SARS-CoV-2 from oral and nasopharyngeal swabs.
Keywords: COVID19; SARS-CoV2; mass spectrometry; severe acute respiratory syndrome coronavirus 2.