tetano
Editor, Senior Moderator
Viruses
. 2024 Nov 22;16(12):1818.
doi: 10.3390/v16121818. Genotypic Clustering of H5N1 Avian Influenza Viruses in North America Evaluated by Ordination Analysis
Patil Tawidian[SUP] 1 2 [/SUP], Mia K Torchetti[SUP] 3 [/SUP], Mary L Killian[SUP] 3 [/SUP], Kristina Lantz[SUP] 3 [/SUP], Krista E Dilione[SUP] 4 [/SUP], Jourdan M Ringenberg[SUP] 4 [/SUP], Sarah N Bevins[SUP] 4 [/SUP], Julianna B Lenoch[SUP] 4 [/SUP], Hon S Ip[SUP] 1 [/SUP]
Affiliations
The introduction of HPAI H5N1 clade 2.3.4.4b viruses to North America in late 2021 resulted in avian influenza outbreaks in poultry, mortality events in many wild bird species, and spillovers into many mammalian species. Reassortment events with North American low-pathogenic virus were identified as early as February 2022 and over 100 genotypes have been characterized. Such diversity increases the complexity and time required for monitoring virus evolution. Here, we performed ordination and clustering analyses on sequence data from H5N1 viruses identified in North America between January 2020 and December 2023 to visualize the genotypic diversity of viruses in poultry and wildlife populations. Our results reveal that ordination- and cluster-based approaches can complement traditional phylogenetic analyses specifically for the preliminary assignment of H5N1 viruses to genotypic groups or to identify novel genotypes. Our study expands current knowledge on the genotypic diversity of H5N1 viruses in North America and describes a rapid approach for early virus genotype assignment.
Keywords: H5N1 genotypes; H5N1 virus; avian influenza; high pathogenic influenza virus (HPAI); virus nomenclature; virus reassortment.
. 2024 Nov 22;16(12):1818.
doi: 10.3390/v16121818. Genotypic Clustering of H5N1 Avian Influenza Viruses in North America Evaluated by Ordination Analysis
Patil Tawidian[SUP] 1 2 [/SUP], Mia K Torchetti[SUP] 3 [/SUP], Mary L Killian[SUP] 3 [/SUP], Kristina Lantz[SUP] 3 [/SUP], Krista E Dilione[SUP] 4 [/SUP], Jourdan M Ringenberg[SUP] 4 [/SUP], Sarah N Bevins[SUP] 4 [/SUP], Julianna B Lenoch[SUP] 4 [/SUP], Hon S Ip[SUP] 1 [/SUP]
Affiliations
- PMID: 39772128
- PMCID: PMC11680268
- DOI: 10.3390/v16121818
The introduction of HPAI H5N1 clade 2.3.4.4b viruses to North America in late 2021 resulted in avian influenza outbreaks in poultry, mortality events in many wild bird species, and spillovers into many mammalian species. Reassortment events with North American low-pathogenic virus were identified as early as February 2022 and over 100 genotypes have been characterized. Such diversity increases the complexity and time required for monitoring virus evolution. Here, we performed ordination and clustering analyses on sequence data from H5N1 viruses identified in North America between January 2020 and December 2023 to visualize the genotypic diversity of viruses in poultry and wildlife populations. Our results reveal that ordination- and cluster-based approaches can complement traditional phylogenetic analyses specifically for the preliminary assignment of H5N1 viruses to genotypic groups or to identify novel genotypes. Our study expands current knowledge on the genotypic diversity of H5N1 viruses in North America and describes a rapid approach for early virus genotype assignment.
Keywords: H5N1 genotypes; H5N1 virus; avian influenza; high pathogenic influenza virus (HPAI); virus nomenclature; virus reassortment.