tetano
Editor, Senior Moderator
Viruses
. 2020 Jun 28;12(7):E699.
doi: 10.3390/v12070699.
Saxifraga spinulosa-Derived Components Rapidly Inactivate Multiple Viruses Including SARS-CoV-2
Yohei Takeda[SUP] 1 [/SUP], Toshihiro Murata[SUP] 2 [/SUP], Dulamjav Jamsransuren[SUP] 3 [/SUP], Keisuke Suganuma[SUP] 1 4 [/SUP], Yuta Kazami[SUP] 3 [/SUP], Javzan Batkhuu[SUP] 5 [/SUP], Duger Badral[SUP] 6 [/SUP], Haruko Ogawa[SUP] 3 [/SUP]
AffiliationsExpand
Abstract
Novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus (IAV), and norovirus (NV) are highly contagious pathogens that threaten human health. Here we focused on the antiviral potential of the medicinal herb, Saxifraga spinulosa (SS). Water-soluble extracts of SS were prepared, and their virus-inactivating activity was evaluated against the human virus pathogens SARS-CoV-2 and IAV; we also examined virucidal activity against feline calicivirus and murine norovirus, which are surrogates for human NV. Among our findings, we found that SS-derived gallocatechin gallate compounds were capable of inactivating all viruses tested. Interestingly, a pyrogallol-enriched fraction (Fr 1C) inactivated all viruses more rapidly and effectively than did any of the component compounds used alone. We found that 25 ?g/mL of Fr 1C inactivated >99.6% of SARS-CoV-2 within 10 s (reduction of ≥2.33 log[SUB]10[/SUB] TCID[SUB]50[/SUB]/mL). Fr 1C resulted in the disruption of viral genomes and proteins as determined by gel electrophoresis, electron microscopy, and reverse transcription-PCR. Taken together, our results reveal the potential of Fr 1C for development as a novel antiviral disinfectant.
Keywords: SARS-CoV-2; Saxifraga spinulosa; medicinal herb; pandemic; pyrogallol-enriched fraction; virus disinfectant.
. 2020 Jun 28;12(7):E699.
doi: 10.3390/v12070699.
Saxifraga spinulosa-Derived Components Rapidly Inactivate Multiple Viruses Including SARS-CoV-2
Yohei Takeda[SUP] 1 [/SUP], Toshihiro Murata[SUP] 2 [/SUP], Dulamjav Jamsransuren[SUP] 3 [/SUP], Keisuke Suganuma[SUP] 1 4 [/SUP], Yuta Kazami[SUP] 3 [/SUP], Javzan Batkhuu[SUP] 5 [/SUP], Duger Badral[SUP] 6 [/SUP], Haruko Ogawa[SUP] 3 [/SUP]
AffiliationsExpand
- PMID: 32605306
- DOI: 10.3390/v12070699
Abstract
Novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus (IAV), and norovirus (NV) are highly contagious pathogens that threaten human health. Here we focused on the antiviral potential of the medicinal herb, Saxifraga spinulosa (SS). Water-soluble extracts of SS were prepared, and their virus-inactivating activity was evaluated against the human virus pathogens SARS-CoV-2 and IAV; we also examined virucidal activity against feline calicivirus and murine norovirus, which are surrogates for human NV. Among our findings, we found that SS-derived gallocatechin gallate compounds were capable of inactivating all viruses tested. Interestingly, a pyrogallol-enriched fraction (Fr 1C) inactivated all viruses more rapidly and effectively than did any of the component compounds used alone. We found that 25 ?g/mL of Fr 1C inactivated >99.6% of SARS-CoV-2 within 10 s (reduction of ≥2.33 log[SUB]10[/SUB] TCID[SUB]50[/SUB]/mL). Fr 1C resulted in the disruption of viral genomes and proteins as determined by gel electrophoresis, electron microscopy, and reverse transcription-PCR. Taken together, our results reveal the potential of Fr 1C for development as a novel antiviral disinfectant.
Keywords: SARS-CoV-2; Saxifraga spinulosa; medicinal herb; pandemic; pyrogallol-enriched fraction; virus disinfectant.