tetano
Editor, Senior Moderator
Water Res
. 2021 Apr 22;198:117183.
doi: 10.1016/j.watres.2021.117183. Online ahead of print.
Several forms of SARS-CoV-2 RNA can be detected in wastewaters: Implication for wastewater-based epidemiology and risk assessment
S Wurtzer[SUP] 1 [/SUP], P Waldman[SUP] 2 [/SUP], A Ferrier-Rembert[SUP] 3 [/SUP], G Frenois-Veyrat[SUP] 3 [/SUP], J M Mouchel[SUP] 2 [/SUP], M Boni[SUP] 3 [/SUP], Y Maday[SUP] 4 [/SUP], OBEPINE consortium; V Marechal[SUP] 5 [/SUP], L Moulin[SUP] 6 [/SUP]
Affiliations
Abstract
The ongoing global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a public health emergency of international concern. Although SARS-CoV-2 is considered to be mainly transmitted by inhalation of contaminated droplets and aerosols, SARS-CoV-2 is also detected in human feces and to a less extent in urine, and in raw wastewaters (to date viral RNA only) suggesting that other routes of infection may exist. Monitoring SARS-CoV-2 genomes in wastewaters has been proposed as a complementary approach for tracing the dynamics of virus transmission within human population connected to wastewater network. The understanding on SARS-CoV-2 transmission through wastewater surveillance, the development of epidemic modeling and the evaluation of SARS-CoV-2 transmission from contaminated wastewater are largely limited by our knowledge on viral RNA genome persistence and virus infectivity preservation in such an environment. Using an integrity based RT-qPCR assay this study led to the discovery that SARS-CoV-2 RNA can persist under several forms in wastewaters, which provides important information on the presence of SARS-CoV-2 in raw wastewaters and associated risk assessment.
Keywords: Infectious risk; Particle integrity; Quantification; SARS-CoV-2; Wastewater.
. 2021 Apr 22;198:117183.
doi: 10.1016/j.watres.2021.117183. Online ahead of print.
Several forms of SARS-CoV-2 RNA can be detected in wastewaters: Implication for wastewater-based epidemiology and risk assessment
S Wurtzer[SUP] 1 [/SUP], P Waldman[SUP] 2 [/SUP], A Ferrier-Rembert[SUP] 3 [/SUP], G Frenois-Veyrat[SUP] 3 [/SUP], J M Mouchel[SUP] 2 [/SUP], M Boni[SUP] 3 [/SUP], Y Maday[SUP] 4 [/SUP], OBEPINE consortium; V Marechal[SUP] 5 [/SUP], L Moulin[SUP] 6 [/SUP]
Affiliations
- PMID: 33962244
- DOI: 10.1016/j.watres.2021.117183
Abstract
The ongoing global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a public health emergency of international concern. Although SARS-CoV-2 is considered to be mainly transmitted by inhalation of contaminated droplets and aerosols, SARS-CoV-2 is also detected in human feces and to a less extent in urine, and in raw wastewaters (to date viral RNA only) suggesting that other routes of infection may exist. Monitoring SARS-CoV-2 genomes in wastewaters has been proposed as a complementary approach for tracing the dynamics of virus transmission within human population connected to wastewater network. The understanding on SARS-CoV-2 transmission through wastewater surveillance, the development of epidemic modeling and the evaluation of SARS-CoV-2 transmission from contaminated wastewater are largely limited by our knowledge on viral RNA genome persistence and virus infectivity preservation in such an environment. Using an integrity based RT-qPCR assay this study led to the discovery that SARS-CoV-2 RNA can persist under several forms in wastewaters, which provides important information on the presence of SARS-CoV-2 in raw wastewaters and associated risk assessment.
Keywords: Infectious risk; Particle integrity; Quantification; SARS-CoV-2; Wastewater.