tetano
Editor, Senior Moderator
Yonsei Med J
. 2021 Jul;62(7):584-592.
doi: 10.3349/ymj.2021.62.7.584.
Neutralizing Antibody Responses to SARS-CoV-2 in Korean Patients Who Have Recovered from COVID-19
Yoon Jung Kim[SUP] #[/SUP][SUP] 1 [/SUP], Joon Yong Bae[SUP] #[/SUP][SUP] 2 [/SUP], Sohyun Bae[SUP] 1 [/SUP], Soyoon Hwang[SUP] 1 [/SUP], Ki Tae Kwon[SUP] 1 [/SUP], Hyun Ha Chang[SUP] 1 [/SUP], Won Kee Lee[SUP] 3 [/SUP], Chunguang Cui[SUP] 2 [/SUP], Gee Eun Lee[SUP] 2 [/SUP], Shin Woo Kim[SUP] 4 [/SUP], Man Seong Park[SUP] 5 [/SUP]
Affiliations
Abstract
Purpose: Neutralizing antibodies (NAbs) have been considered effective in preventing and treating viral infections. However, until now, the duration and clinical implications of antibody-mediated nature immunity in Koreans have remained unknown. Therefore, we examined NAbs levels and clinical characteristics in recovered coronavirus disease 2019 (COVID-19) patients.
Materials and methods: Blood samples were collected from 143 adult patients who had been diagnosed with and had recovered from COVID-19 from February to March in 2020 at a tertiary-care university-affiliated hospital in Daegu, Korea. A plaque reduction neutralization test was conducted to analyze NAb titers. Individualized questionnaires were used to identify patient clinical information.
Results: The median number of days from symptom onset to the blood collection date was 109.0 (104.0; 115.0). The NAb titers ranged from 10 to 2560. The median NAb titer value was 40. Of the 143 patients, 68 (47.6%) patients had NAb titers ≥80, and 31 (21.7%) patients had NAb titers ≥160. The higher the age or disease severity, the higher the NAb titer. In univariate logistic regression, statistically significant predictors of high NAb titers (≥80) were age, myalgia, nausea or vomiting, dyspnea, and disease severity (p<0.05). Multivariable logistic regression showed that age ≥50 years (p=0.013) and moderate or higher disease severity (p<0.001) were factors associated with high NAb titers (≥80). None of the patients had reinfection of COVID-19.
Conclusion: All recovered patients were found to have NAbs regardless of the NAb titers maintained by natural immunity. Age and disease severity during COVID-19 infection were associated with high NAb titers.
Keywords: COVID-19; SARS-CoV-2; immunity; neutralization assay; neutralizing antibodies.
. 2021 Jul;62(7):584-592.
doi: 10.3349/ymj.2021.62.7.584.
Neutralizing Antibody Responses to SARS-CoV-2 in Korean Patients Who Have Recovered from COVID-19
Yoon Jung Kim[SUP] #[/SUP][SUP] 1 [/SUP], Joon Yong Bae[SUP] #[/SUP][SUP] 2 [/SUP], Sohyun Bae[SUP] 1 [/SUP], Soyoon Hwang[SUP] 1 [/SUP], Ki Tae Kwon[SUP] 1 [/SUP], Hyun Ha Chang[SUP] 1 [/SUP], Won Kee Lee[SUP] 3 [/SUP], Chunguang Cui[SUP] 2 [/SUP], Gee Eun Lee[SUP] 2 [/SUP], Shin Woo Kim[SUP] 4 [/SUP], Man Seong Park[SUP] 5 [/SUP]
Affiliations
- PMID: 34164955
- DOI: 10.3349/ymj.2021.62.7.584
Abstract
Purpose: Neutralizing antibodies (NAbs) have been considered effective in preventing and treating viral infections. However, until now, the duration and clinical implications of antibody-mediated nature immunity in Koreans have remained unknown. Therefore, we examined NAbs levels and clinical characteristics in recovered coronavirus disease 2019 (COVID-19) patients.
Materials and methods: Blood samples were collected from 143 adult patients who had been diagnosed with and had recovered from COVID-19 from February to March in 2020 at a tertiary-care university-affiliated hospital in Daegu, Korea. A plaque reduction neutralization test was conducted to analyze NAb titers. Individualized questionnaires were used to identify patient clinical information.
Results: The median number of days from symptom onset to the blood collection date was 109.0 (104.0; 115.0). The NAb titers ranged from 10 to 2560. The median NAb titer value was 40. Of the 143 patients, 68 (47.6%) patients had NAb titers ≥80, and 31 (21.7%) patients had NAb titers ≥160. The higher the age or disease severity, the higher the NAb titer. In univariate logistic regression, statistically significant predictors of high NAb titers (≥80) were age, myalgia, nausea or vomiting, dyspnea, and disease severity (p<0.05). Multivariable logistic regression showed that age ≥50 years (p=0.013) and moderate or higher disease severity (p<0.001) were factors associated with high NAb titers (≥80). None of the patients had reinfection of COVID-19.
Conclusion: All recovered patients were found to have NAbs regardless of the NAb titers maintained by natural immunity. Age and disease severity during COVID-19 infection were associated with high NAb titers.
Keywords: COVID-19; SARS-CoV-2; immunity; neutralization assay; neutralizing antibodies.