tetano
Editor, Senior Moderator
Zool Res
. 2021 Nov 18;42(6):833-843.
doi: 10.24272/j.issn.2095-8137.2021.334.
The high diversity of SARS-CoV-2-related coronaviruses in pangolins alters potential ecological risks
Min-Sheng Peng[SUP] 1 2 3 [/SUP], Jian-Bo Li[SUP] 1 2 [/SUP], Zheng-Fei Cai[SUP] 4 [/SUP], Hang Liu[SUP] 1 2 [/SUP], Xiaolu Tang[SUP] 5 [/SUP], Ruochen Ying[SUP] 5 [/SUP], Jia-Nan Zhang[SUP] 6 [/SUP], Jia-Jun Tao[SUP] 6 [/SUP], Ting-Ting Yin[SUP] 1 [/SUP], Tao Zhang[SUP] 4 [/SUP], Jing-Yang Hu[SUP] 4 [/SUP], Ru-Nian Wu[SUP] 1 [/SUP], Zhong-Yin Zhou[SUP] 1 [/SUP], Zhi-Gang Zhang[SUP] 4 [/SUP], Li Yu[SUP] 4 [/SUP], Yong-Gang Yao[SUP] 2 7 8 [/SUP], Zheng-Li Shi[SUP] 9 [/SUP], Xue-Mei Lu[SUP] 1 2 10 [/SUP], Jian Lu[SUP] 11 [/SUP], Ya-Ping Zhang[SUP] 1 2 7 4 12 [/SUP]
Affiliations
Abstract
in English, Chinese
Understanding the zoonotic origin and evolution history of SARS-CoV-2 will provide critical insights for altering and preventing future outbreaks. A significant gap remains for the possible role of pangolins as a reservoir of SARS-CoV-2 related coronaviruses (SC2r-CoVs). Here, we screened SC2r-CoVs in 172 samples from 163 pangolin individuals of four species, and detected positive signals in muscles of four M. javanica and, for the first time, one M. pentadactyla. Phylogeographic analysis of pangolin mitochondrial DNA traced their origins from Southeast Asia. Using in-solution hybridization capture sequencing, we assembled a partial pangolin SC2r-CoV (pangolin-CoV) genome sequence of 22 895 bp from the M. pentadactyla sample. Phylogenetic analyses revealed MP20 was very closely related to pangolin-CoVs that were identified in Malayan pangolins seized by Guangxi Customs. A genetic contribution of bat coronavirus to pangolin-CoVs via recombination was indicated. Our analysis revealed that the genetic diversity of pangolin-CoVs is substantially higher than previously anticipated. Given the potential infectivity of pangolin-CoVs, the high genetic diversity of pangolin-CoVs alerts the ecological risk of zoonotic evolution and transmission of pathogenic SC2r-CoVs.
Keywords: Diversity; Pangolin; Recombination; SARS-CoV-2; Sequencing; mtDNA.
. 2021 Nov 18;42(6):833-843.
doi: 10.24272/j.issn.2095-8137.2021.334.
The high diversity of SARS-CoV-2-related coronaviruses in pangolins alters potential ecological risks
Min-Sheng Peng[SUP] 1 2 3 [/SUP], Jian-Bo Li[SUP] 1 2 [/SUP], Zheng-Fei Cai[SUP] 4 [/SUP], Hang Liu[SUP] 1 2 [/SUP], Xiaolu Tang[SUP] 5 [/SUP], Ruochen Ying[SUP] 5 [/SUP], Jia-Nan Zhang[SUP] 6 [/SUP], Jia-Jun Tao[SUP] 6 [/SUP], Ting-Ting Yin[SUP] 1 [/SUP], Tao Zhang[SUP] 4 [/SUP], Jing-Yang Hu[SUP] 4 [/SUP], Ru-Nian Wu[SUP] 1 [/SUP], Zhong-Yin Zhou[SUP] 1 [/SUP], Zhi-Gang Zhang[SUP] 4 [/SUP], Li Yu[SUP] 4 [/SUP], Yong-Gang Yao[SUP] 2 7 8 [/SUP], Zheng-Li Shi[SUP] 9 [/SUP], Xue-Mei Lu[SUP] 1 2 10 [/SUP], Jian Lu[SUP] 11 [/SUP], Ya-Ping Zhang[SUP] 1 2 7 4 12 [/SUP]
Affiliations
- PMID: 34766482
- DOI: 10.24272/j.issn.2095-8137.2021.334
Abstract
in English, Chinese
Understanding the zoonotic origin and evolution history of SARS-CoV-2 will provide critical insights for altering and preventing future outbreaks. A significant gap remains for the possible role of pangolins as a reservoir of SARS-CoV-2 related coronaviruses (SC2r-CoVs). Here, we screened SC2r-CoVs in 172 samples from 163 pangolin individuals of four species, and detected positive signals in muscles of four M. javanica and, for the first time, one M. pentadactyla. Phylogeographic analysis of pangolin mitochondrial DNA traced their origins from Southeast Asia. Using in-solution hybridization capture sequencing, we assembled a partial pangolin SC2r-CoV (pangolin-CoV) genome sequence of 22 895 bp from the M. pentadactyla sample. Phylogenetic analyses revealed MP20 was very closely related to pangolin-CoVs that were identified in Malayan pangolins seized by Guangxi Customs. A genetic contribution of bat coronavirus to pangolin-CoVs via recombination was indicated. Our analysis revealed that the genetic diversity of pangolin-CoVs is substantially higher than previously anticipated. Given the potential infectivity of pangolin-CoVs, the high genetic diversity of pangolin-CoVs alerts the ecological risk of zoonotic evolution and transmission of pathogenic SC2r-CoVs.
Keywords: Diversity; Pangolin; Recombination; SARS-CoV-2; Sequencing; mtDNA.